Anti-nmt55 / p54nrb antibody ab70335 from Abcam

Product Specs
ItemAnti-nmt55 / p54nrb antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab70335
Antibody TypePrimary
Quantity100 UL
HostRabbit
Conjugate/TagUnconjugated
IsotypeIgG
ClonalityPolyclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Gene NameNONO
ReactivityHuman, Mouse, Rat
Targetnmt55 / p54nrb
Concentration0.2 mg/ml

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) circ-hnRNPU inhibits NONO-mediated c-Myc transactivation and mRNA stabilization essential for glycosylation and cancer progressionJournal of Experimental & Clinical Cancer ResearchNovember 23, 2023Hongjun Li, Wanju Jiao, Jiyu Song, Jianqun Wang, Guo Chen, Dan Li, Xiaojing Wang, Banghe Bao, Xinyi Du, Yang Cheng, et al.Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, Temecula, CA), using antibodies specific for NONO (ab70335) or Flag-tag (ab45766, Abcam Inc.) and protein A-Sepharose beads (Santa Cruz Biotechnology) at 4 °C

(2) Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensatesScience AdvancesOctober 18, 2023Brian A. Lewis, Subhendu Kumar Das, Rajiv Kumar Jha, David Levenssc-55492 AF488; Alexa Fluor 594, Santa Cruz Biotechnology, sc-55492 AF594), NONO (anti-nmnt/p54, Abcam, ab70335), SFPQ (Abcam, ab177149), anti-BrU antibody (Abcam, ab152095), CDK9 antibody (Bethyl A303-493A), FUS

(3) Stabilization of SAMHD1 by NONO is crucial for Ara-C resistance in AMLCell death & diseaseJuly 8, 2022Zhang F, Sun J, Tang X, Liang Y, Jiao Q, Yu B, Dai Z, Yuan X, Li J, Yan J, et al.used for western blotting were purchased from Proteintech (Wuhan, China). The antibodies against NONO (ab70335) and SAMHD1 (ab264335) used for co-IP were obtained from Abcam (Cambridge, UK). Reverse transcription

(4) Identification and targeting of G-quadruplex structures in MALAT1 long non-coding RNANucleic Acids ResearchJanuary 11, 2022Xi Mou, Shiau Wei Liew, Chun Kit Kwokand quantified by ImageJ. Primary antibodies used are as follows: Anti-nmt55/p54nrb antibody (Abcam ab70335), Anti-GAPDH (Bio-station limited sc-32233). RESULTS Identification and characterization of G-quadruplex

(5) Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retentionTheranosticsJanuary 1, 2022Wang X, Han M, Wang S, Sun Y, Zhao W, Xue Z, Liang X, Huang B, Li G, Chen A, et al.radish peroxidase and the substrate DAB. The following primary antibodies were used: rabbit anti-NONO (ab70335, 1:200; Abcam; Waltham, MA, USA), rabbit anti-Ki67 (GB13030-2, 1:1,000; Servicebio; Wuhan, China) and

(6) Speckles and paraspeckles coordinate to regulate HSV-1 genes transcriptionCommunications biologyOctober 21, 2021Li K, Wang Z.proteins were detected by western blotting with antibodies against PSPC1 (Abcam, ab104238), P54nrb (Abcam, ab70335), P300 (Abcam, ab59240), CBP (Abcam, ab50702), SRSF2 (Santa Cruz Biotechnology, sc-10252), EZH2 (Abcam,

(7) Knockout of the NONO Gene Inhibits Neointima Formation in a Mouse Model of Vascular InjuryArteriosclerosis, thrombosis, and vascular biologyApril 1, 2021Xu (徐兴丽) X, Xu (徐兴华) X, Mao (毛洋) Y, Lu (卢琳) L, Ma (马静) J, Zheng (郑腾飞) T, Zhang (张杰) J, Zhang (章萌) M, Meng (孟霖霖) L, Ma (马连越) L, et al.Solarbio) in PBS for 1 hour at room temperature and incubated overnight at 4 °C with anti-NONO (Abcam; ab70335, 2 μg/mL) and antiSM22α (smooth muscle protein 22-α; Abcam; ab212857, 1 μg/mL) antibody mixture in PBST.

(8) The RNA-binding protein SFPQ preserves long-intron splicing and regulates circRNA biogenesis in mammalseLifeJanuary 21, 2021Lotte Victoria Winther Stagsted, Eoghan Thomas O'Leary, Karoline Kragh Ebbesen, Thomas Birkballe Hansendebris were pelleted by centrifugation at 13,000 RPM for 20 min. Antibody to SFPQ (P2860 Sigma), NONO (ab70335 Abcam), or FLAG epitope (Mock IP, F1804 Sigma) was added to supernatant (2.5 μg) and incubated for 4

(9) Paraspeckle Promotes Hepatocellular Carcinoma Immune Escape by Sequestering IFNGR1 mRNACellular and molecular gastroenterology and hepatologyJanuary 1, 2021Zan J, Zhao X, Deng X, Ding H, Wang B, Lu M, Wei Z, Huang Z, Wang S.Signal Technology), phosphorylated STAT1 (pSTAT1) (1:1000, 9167; Cell Signal Technology), NONO (1:1000, ab70335; Abcam), flag (1:2000, ab18230; Abcam), Phospho-(Ser/Thr) (1:500, ab117253; Abcam), and Phospho-Tyrosine

(10) NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNACancer Cell InternationalDecember 1, 2020Hongda Ding, Junpeng Liu, Caibin Wang, Yang SuSDS-PAGE and immunoblotting, as previously described [24]. Primary antibodies against NONO (1:500; ab70335, Abcam, USA), ACLY (1:500; ab40793, Abcam, USA), Insulin like growth factor 2 mRNA binding protein 1

(11) SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assembliesNucleic acids researchNovember 18, 2020Toki N, Takahashi H, Sharma H, Valentine MNZ, Rahman FM, Zucchelli S, Gustincich S, Carninci P.marker antibodies were tested as follows; anti-SC35 (S4045, Sigma-Aldrich), anti-nmt55/p54nrb (NONO, ab70335, Abcam), anti-ILF3 (ab133354, Abcam), anti-EF1A (sc-21758, SantaCruz), anti-ATP5 (mitochondria, ab14748,

(12) H. pylori infection alters repair of DNA double-strand breaks via SNHG17Journal of Clinical InvestigationJune 15, 2020Taotao Han et al.(nanoLC-LTQOrbitrap XL, Thermo Fisher Scientific) or hybridization with antiNONO antibody (Abcam, catalog ab70335). RIP and UV-RIP. RIP was performed using a Magna RIP RNA-Binding Protein Immunoprecipitation Kit (MilliporeSigma)

(13) BCL9 provides multi-cellular communication properties in colorectal cancer by interacting with paraspeckle proteinsNature communicationsJanuary 7, 2020Jiang M, Kang Y, Sewastianik T, Wang J, Tanton H, Alder K, Dennis P, Xin Y, Wang Z, Liu R, et al.anti-BCL9 (1:500 dilution, ab37305 Abcam, 6109 generated in New England Biolabs), NONO (1:500 dilution, ab70335, Abcam), SFPQ (1:500 dilution, ab38148), ILF2 (1:500 dilution, H00003608-D01, Abnova), or β-catenin

(14) Kinetic and subcellular analysis of PS-ASO/protein interactions with P54nrb and RNase H1Nucleic acids researchNovember 18, 2019Vickers TA, Rahdar M, Prakash TP, Crooke ST.for 30 min, and incubated in blocking buffer with primary antibody NanoLuc pAb, Promega; P54nrb pAb, ab70335 ABCAM) at room temperature for 2 h. After washing three times with washing buffer (0.1% Tween-20 in

(15) The FUS-DDIT3 Interactome in Myxoid LiposarcomaNeoplasia (New York, N.Y.)August 1, 2019Yu JSE, Colborne S, Hughes CS, Morin GB, Nielsen TO.normal mouse or rabbit IgG (Santa Cruz), DDIT3 (L63F7) (Cell Signaling Technology, #2895), NONO (Abcam, ab70335), PSPC1 (Abcam, ab104238), and SFPQ (Novus Biologicals, NB-100-61045). Antibodies were first incubated

(16) Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation TransitionMolecular cellJune 6, 2019Modic M, Grosch M, Rot G, Schirge S, Lepko T, Yamazaki T, Lee FCY, Rusha E, Shaposhnikov D, Palo M, et al.Antibody,Thermo Fisher Scientific,Cat no. MA1-25325; RRID: AB_2186931 NONO polyclonal Antibody,Abcam,Cat no. ab70335; RRID: AB_1269576 CPEB1 polyclonal Antibody,Cell Signaling,Cat no. 13583 PSPC1 rabbit polyclonal antibody,"Naganuma

(17) Ets-1 promoter-associated noncoding RNA regulates the NONO/ERG/Ets-1 axis to drive gastric cancer progressionOncogeneAugust 1, 2018Li D, Chen Y, Mei H, Jiao W, Song H, Ye L, Fang E, Wang X, Yang F, Huang K, et al.Western blotting was performed as previously reported [12, 13, 49–51], with antibodies specific for NONO (ab70335), ERG (ab92513), Ets-1 (ab26096), FLAG (ab45766), HA (ab9110, Abcam Inc., Cambridge, MA), histone H3

(18) Apoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing FactorsCancer cellJuly 9, 2018Pavlyukov MS, Yu H, Bastola S, Minata M, Shender VO, Lee Y, Zhang S, Wang J, Komarova S, Wang J, et al.,Cell Signaling,Cat# 4410 anti-mouse IgG-Horseradish peroxidase,GE Healthcare,Cat# NXA931 anti-NONO,Abcam,Cat# ab70335 anti-phospho-H2AX,EMD Millipore,Cat# 05-636 anti-PRPF8,Abcam,Cat# ab185547 anti-rabbit IgG-Alexa

(19) Neat1 regulates oxidized low-density lipoprotein-induced inflammation and lipid uptake in macrophages via paraspeckle formationMolecular Medicine ReportsDecember 7, 2017Ning Huang‑Fu, Jing‑Song Cheng, Yong Wang, Zhen‑Wei Li, Sheng‑Huang Wangdifferentiation 36 (CD36; 1:1,000; ab133625); rabbit anti-LOX-1 (1:1,000; ab60178); rabbit anti-NONO (1:1,000; ab70335); rabbit anti-SFPQ (1:1,000; ab38148) (all from Abcam, Shanghai, China); goat anti-rabbit (1:5,000;

(20) Genome-wide mapping of infection-induced SINE RNAs reveals a role in selective mRNA exportNucleic Acids ResearchJune 2, 2017John Karijolich, Yang Zhao, Ravi Alla, Britt Glaunsingertransferred to a polyvinylidene difluoride membrane, and incubated with the indicated antibodies (anti-p54nrb ab70335, Abcam; anti-GAPDH ab8245, Abcam). Western blots assays were developed with HRP-conjugated Protein G

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Anti-nmt55 / p54nrb antibody from Abcam

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Anti-nmt55 / p54nrb antibody from Abcam

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